Liverpool forward Luis Suarez dives to win penalty as Uruguay beat Argentina
The Liverpool forward tumbled in the area to win a spot-kick which he then stepped up to convert
But the bite-ban striker and his international team-mates missed out on automatic qualification from the South American zone on goal difference.
The 1930 and 1950 World Cup winners must now playoff against Jordan after finishing fifth in the CONMEBOL table.
The visitors were short of their their star man as Barcelona forward Messi missed the game through injury.
Suarez played a part in all of his side's goals as they edged out their South American rivals 3-2 at the Estadio Centenario in Montevideo.
The Anfield forward's free-kick was flicked on by West Brom's Diego Lugano for Cristian Rodriguez to slot home before Maxi Rodriguez levelled.
Suarez then tumbled in the box to earn a contentious penalty, dusting himself off to score the spot-kick himself (see below).
Rodriguez equalised once again just before half time but the Liverpool man - who has been in fine form since return from his 10-match bite-ban - was instrumental again as Edinson Cavani sealed the win.
The Paris Saint-Germain striker converted a Suarez pass to ensure the win, although Ecuador grabbed the last CONMEBOL qualifying spot alongside Argentina, Colombia and Chile.